Ph.D., Yale Unversity, Molecular Biophysics and Biochemisty
Postdoc, University of Wisconsin-Madison, Cell Biology
NRSA Predoctoral Fellowship, American Cancer Society Postdoctoral Fellowship, Helen Hay Whitney Postdoctoral Fellowship, K99/R00 Pathway to Independence Grant from NIGMS, R01 from NIGMS
In the Miller Lab, we are investigating how cytokinesis works in intact epithelial tissues, where the dividing cell gives and receives inputs from its neighboring cells. We know that localized activation of RhoA, a small GTPase that promotes actomyosin contractility, is required both for cytokinesis and for proper cell-cell junctions. We don’t understand the signaling pathways that lead to proper localized Rho GTPase activity. Therefore, our lab is focused on characterizing novel mechanisms by which localized Rho GTPase activity is regulated both at the cell division site and at cell-cell junctions. Using live high-resolution confocal microscopy in Xenopus laevis (frog) embryos where potential players in Rho GTPase regulation have been perturbed, we are dissecting the mechanisms that regulate cytokinesis and cell-cell junctions in space and time.
Bement, W.M., Leda, M., Moe, A., Kita, A.M., Larson, M.E., Golding, A., Pfeuti, C., Su, K-C., Miller, A.L., Goryachev, A.B., and von Dassow, G. (2015). "Activator–inhibitor coupling between Rho signalling and actin assembly makes the cell cortex an excitable medium." Nature Cell Biol. In Press.
Breznau, E.B., Semack, A., Higashi, T., and Miller, A.L. (2015). MgcRacGAP restricts active RhoA at the cytokinetic furrow and both RhoA and Rac1 at cell-cell junctions in epithelial cells. Molecular Biology of the Cell, 26:2439-2455.
Reyes, C.C., Jin, M., Breznau, E.B., Espino, R, Delgado-Gonzalo, R., Goryachev, A.B., and Miller, A.L. (2014). “Anillin Regulates Cell-Cell Junction Integrity by Organizing Junctional Accumulation of Rho-GTP and Actomyosin.” Current Biology, 24:1263-1270.
Miller AL (2011). " The contractile ring." Current Biology 21:R976-978.
Vaughan, E.M., Miller, A.L., and Bement W.M. (2011). "Control of Local Rho GTPase Crosstalk by Abr." Current Biology 21: 270-277.
Miller, A.L. and Bement, W.M. (2009). "Cell Division: The need for speed." Current Biology 19:R1071-1073.
von Dassow, G., Verbrugghe, K.J.C., Miller, A.L., Sider, J.R., and Bement, W.M. (2009). "Action at a distance during cytokinesis."Journal of Cell Biology 187:831-845.
Woolner, S., Miller, A.L., and Bement, W.M. (2009). "Imaging the cytoskeleton in live Xenopus laevis embryos." Methods in Molecular Biology: Cytoskeleton Methods and Protocols 586:23-39.
Clark, A.G., Miller, A.L., Vaughan, E., Yu, H-Y.E., Penkert, R., Bement, W.M. (2009). "Integration of single and multicellular wound responses." Current Biology 19:1-7.
Miller, A.L. and Bement, W.M. (2009). "Regulation of cytokinesis by Rho GTPase flux." Nature Cell Biology 11:71-77.