"The logic and topology of experimentally verifiable statements." Presentation given by Gabriele Carcassi at the 33rd Summer Conference on Topology and its Applications, Western Kentucky University, July 18, 2018. Slides available at http://assumptionsofphysics.org/20180718-33rdTopology-Slides.pdf.
This poster presentation presented data on bilateral kidney stimulation in diabetic-model rats.
This poster presentation summarized our experiments using electrical stimulation on one kidney.
This poster presented preliminary data on modulating kidney function with electrical stimulation.
In this paper we demonstrated that electrical stimulation of renal nerves can modulate the kidney's control over glucose filtration.
Glioblastoma multiformae (GBM) is the most aggressive type of malignant brain tumor with complex molecular profile. In subsets of GBM, increased expression of Na+/H+ exchanger, NHE9, is a potent driver of tumor progression and is associated with decreased patient survival. In glia, NHE9 is localized to sorting endosomes where it regulates endosomal pH by transporting luminal protons out in exchange for sodium or potassium ions. Overexpression of NHE9 alkalinizes the sorting endosomes and attenuates turnover of epidermal growth factor receptors (EGFRs) thereby increasing persistence of EGFRs on the plasma membrane. EGFR signaling regulates multiple cellular functions including cell growth and division. Downregulation of NHE9 acidifies sorting endosomes limiting EGFR trafficking to the glioblastoma cell membrane and is a potential therapeutic strategy for glioblastomas with NHE9 overexpression. We are conducting pilot studies of a trimodal therapeutic approach, in cell culture experiments. Gold nanoshell-enabled photothermal therapy (NEPTT) is emerging as a promising platform for cancer treatment. Silica-core/gold-shell nanoparticles can absorb radiation in the 700-900 nm range to target cancer cells while leaving the surrounding, healthy tissue unharmed. We have successfully synthesized gold nano-shells on ~200 diameters silica cores. It is observed that endocytic uptake of gold nanoshells is elevated in glioblastoma cells that overexpress NHE9. Herein, we report results from the effects of combinatorial approach using NHE9 downregulation, tyrosine kinase inhibition and NEPTT on glioblastoma cell proliferation.