Our team project is to pilot the functionality of our methods to resolve fundamental questions about the interaction of peony viruses and the complex peony genotypes in our garden. What are the interactions of peony and viral genomes in the UM Peony Garden, and how do we make sense of them? Understanding the presence and interactions of peony viruses is critically important to our work on peony conservation (a key focus on the Peony Garden). There is no relevant literature in this emergent field of landscape-scale genomics.
This pilot $15K proposal would let us demonstrate the project validity, identify and outline unforseen complexities to resolve, and go for larger UM funding (as $60K UM) as well as 6 and 7 figure foundation grants. One critical aspect is the complex data curation needed to make this data analysis ready, and to guide its long term stewardship.
The Peony Garden is a compelling study-site for the initial work, since the history of each of the nearly 700 planting-spots has been recorded since the 1920s.
We thus can have:
a) spatial data of what-is-where to overlay with
b) depth of time on a site since some plants moved internally (this was recorded to the plant) with
c) the increasingly extensive data for molecular markers for peony cultivar-relationships with
d) the historical data about alleged cultivar parentage with
e) genomic determination of silent and evident viruses.