Determination of relationships between phosphoinositide lipids and neoplastic cells
Phosphoinositide lipids are critical for normal animal physiology. These metabolites are low in abundance, and consequently, difficult to detect. Moreover, the phenotypes associated with defects these phosphoinositides are pleiotropic, making it difficult to determine pathways regulated directly by these lipids. We seek to gain insight into of these lipids by using CRISPR/CAS9 technology to generate tagged versions of the lipid kinase responsible for the generation of PI(3,5)P2. The combination of identifying the cellular locations where PIKfyve resides in tandem with phenotypic changes observed upon its acute inhibition will allow us to characterize PI(3,5)P2-dependent pathways operative in both normal and neoplastic cells. Insights gained will be used to design new strategies for manipulating PI(3,5)P2 levels at specific intracellular locations in order to control a subset of pathways regulated by this unique phosphoinositide.
Keynote lecturer at the 44th Symposium on Hormone and Cell Regulation, Mont Ste Odile, France
Roles of PIKfyve and phosphoinositide lipids in cell migration: implications for heart development. Postdoctoral Fellowship
$106,532 grant from the American Heart Association
Published article in eLife