High throughput genetic screening system
CRISPR-Cas9 has been recently introduced as a powerful genomic editing technology. We are adapting and further developing this technology using our existing skills and resources to identify novel regulators in cell function. In this novel system, we will generate CRISPR-Cas9 delivering lentiviruses that carry sgRNA library comprised of oligos either targeting whole genome or a selected group of genes. Each gene will be covered by 3 to 6 different sgRNAs. Our results in different functional selection system will be robustly validated in models that have high translational potential, including but are not limited to novel targets in salivary gland cancer resistance to treatment, or design specifications for optimization of cell growth and differentiation on 3D printed patient-specific regenerative tissue engineered scaffolds.
$1,800,000 grant from the National Institute of Health.