Acute gastroenteritis (AGE) is a common and significant cause of morbidity and mortality in children, and places a substantial health and socioeconomic burden on society. Enrollment in child care centers (CCCs) is a known and significant risk factor for AGE with over 65% of US children attending child care yearly. Despite the clear importance of controlling viral AGE transmission within CCCs, notably from norovirus, there is a gap in our knowledge of effective environmental interventions within CCCs for disrupting transmission of viral AGE. In this project, we will leverage the existing MCRISP system, a network of over 30 child cares with real-time web-based reporting of syndromic illnesses. The overall objective of our proposed work is to develop an environmental fomite decontamination protocol to reduce the incidence of viral AGE among CCC attendees. First, it is critical to establish the patterns by which viruses spread throughout child care centers. In the first phase of the project, we will collect environmental sampling data in a single child care center guided by high-resolution microactivity data taken from video capture of children. In the second phase, we will monitor a large network of CCCs to locate outbreaks of AGE and conduct environmental sampling during these periods to identify fomites that play a key role in transmission. Our proposed work is based in an established, regional network of CCCs, the Michigan Child Care Related Infections Surveillance Program (MCRISP) which conducts real-time web-based reporting of CCC-related illness, enabling the rapid identification on CCC outbreaks.