Manipulating CXCL12-CXCR4 signaling pathway in breast cancer with an experimental & computational approach
The chemokine CXCL12 (SDF-1) is a critical mediator of tumor growth and metastasis in breast cancer. CXCL12 signaling is the integrated output from CXCR4 and decoy receptor CXCR7. To understand how this pathway functions in vitro and in vivo, we will use a combined experimental and computational approach. Via imaging in populations of cells and at single cell resolution, we will detect signaling events in real time to understand relevant signaling dynamics. Intersections of this pathway with cellular metabolism may also be relevant. Using hybrid agent-based computational models, we will integrate molecular, cellular and tissue level events to understand and predict manipulations that may alter these pathways. Through iterative experimental and computational methods, we aim to identify critical mechanisms that can be targeted for breast cancer therapy.
Published in Translational Oncology, 2014
Published in PLoS ONE, 2014
Microfluidic source-sink model reveals effects of biophysically distinct CXCL12-isoforms in breast cancer chemotaxis
Published in Integrative Biology, 2014
Published in PLoS ONE