Advances in microfluidic technologies, coupled with multielectrode arrays and calcium imaging, offer new opportunities to study the networked activity of human neurons. In particular, 3D design of micro/nano fluidic biochips allows the orderly and reproducible generation of networks of cells in culture. These biochips facilitate both electrical and calcium measurement in engineered spatial networks designed to optimize functional connectivity of human neurons. Neurons derived from control individuals and patients suffering from neurological disorders are now routinely generated from fibroblasts. In this project, impediments preventing the systematic study of human neural networks relevant to human brain disorders will be directly addressed. The results of these studies will facilitate analysis of the electrophysiological and networked synaptic activity of neurons derived from controls and patients suffering from epilepsy.
Fibroblast growth factor 2 regulates activity and gene expression of human post-mitotic excitatory neurons
Published in the Journal of Neurochemistry