How bacteria in the human gut recognize, bind to and help digest starches
We aim to develop novel methodologies for labeling and imaging Starch utilization system (Sus) outer-membrane proteins (OMPs) in live Bacteroides thetaiotaomicron cells to investigate their assembly and co-localization in response to starch. Probing the molecular interactions and dynamics of pairs of Sus OMPs will provide mechanistic insight into Sus function, which can be extended to the many related systems that operate in other human gut symbionts during nutrient metabolism. Furthermore, the techniques developed will be broadly applicable to other bacterial and eukaryotic models.
$1,654,079 grant from the Department of Health & Human Services, National Institutes of Health
$186,737 grant from the National Institutes of Health