This project will pursue two stategies:
1) Temporal and spatial control of angiogenic and osteogenic factor gene expression. We developed a novel system for spatial and temporal control of gene expression involving focused ultrasound heating and subsequent activation of genes under the control of a heat shock promoter. Studies will determine if this system can be used to form blood vessels and bone with specific morphologies in vivo.
2) Direct regulation of bone formation using low intensity focused ultrasound. Studies will test the hypothesis that focused low intensity pulsed ultrasound promotes spatially-restricted bone formation through direct mechanical stimulation of cells in and near the ultrasound focus. Studies will measure effects of ultrasound on known mechanotransduction pathways and relate results to osteoblast gene expression and bone formation. In addition, we will determine if regenerated bone morphology can be controlled by applying focused ultrasound to selective regions of the regenerating bone surface.
Published in Biomaterials, 2014
Published in Tissue Engineering Part C Methods, 2014
Spatiotemporal control of vascular endothelial growth factor expression using a heat shock and rapamycin-dependent gene switch
Published in Human Gene Therapy Methods, 2013