Ph.D. Johns Hopkins University. 2004.
Postdoctoral Training - Fred Hutchinson Cancer Research Center 2004-2010.
We study how the cell cycle is regulated to speed up, slow down or stop cell proliferation at the right places and times during development.
Our lab uses tissues of the fruit fly, Drosophila melanogaster to genetically label cells, manipulate cell cycle regulators, and measure proliferation rates in vivo. We are interested in quiescent states, either reversible (as in the case of resting stem cells in the Drosophila intestine) or permanently postmitotic cells as in the case of terminally differentiated cells. Our lab's goal is to identify and understand the barriers to proliferation in quiescent cells to understand why some states of quiescence are readily reversible while others are permanent.
We especially enjoy finding new ways to genetically manipulate the cell cycle to force cells that should be quiescent to actively divide.
O'Keefe D David, Thomas R Sean, Bolin Kelsey, Griggs Ellen, Edgar A Bruce, Buttitta A Laura, "Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters" BMC Genomics 2012, 13:498.
L. Buttitta, A.J. Katzaroff, B.A. Edgar (2010). "A robust cell cycle control mechanism limits E2F-induced proliferation of terminally differentiated cells in vivo." Journal of Cell Biology vol. 189, 981-96.
L. Buttitta and B.A. Edgar (2007). "How size is controlled: From Hippos to Yorkies." Nature Cell Biology Vol. 9, 1225-1227.
L. Buttitta and B.A. Edgar (2007). " Mechanisms coordinating cell cycle exit and terminal differentiation." Current Opinion in Cell Biology Vol. 19, 697-704.
L. Buttitta, A.J. Katzaroff, C. Perez, A. De la Cruz, B.A. Edgar (2007). "A double-assurance mechanism controls cell cycle exit upon terminal differentiation in Drosophila." Developmental Cell Vol. 12, 631-643.
L. Buttitta, R. Mo, C.C. Hui and C.M. Fan (2003). "Interplays of GLI transcription factors and their requirement in mediating SHH-dependent sclerotome induction." Development Vol. 130, 6233-6243.
L. Buttitta, T.S. Tanaka, A. Chen, M.S.H. Ko and C.M. Fan (2003). "Microarray analysis of somitogenesis reveals novel targets of different WNT signaling pathways in the somitic mesoderm." Developmental Biology Vol. 258, 91-104.