Ph.D. Indiana University. 1999.
Amyloid plaques are insoluble protein aggregations that underlie many neurodegenerative disorders such as Alzheimer's, Huntington's, and the prion diseases. Very little is known about amyloid fiber polymerization. Our lab seeks to understand amyloid formation by studying the biogenesis of an amyloid fiber produced by bacteria called curli. Curli fibers produced by E. coli and other enterobacteriaceae members are important determinants of biofilm formation and are proposed to be virulence factors. Our lab uses a variety of biochemical, microscopic, and genetic techniques to elucidate how E. coli assembles these biologically fascinating fibers.
We are also interested in the native function of curli fibers. Curli have been shown to be an integral part of a complex stationary phase developmental pathway in E. coli and Salmonella. This pathway has clear implications for the ability of these bacteria to both survive in nature and to cause disease in humans. Therefore, we are investing the role of curli in coordinating distinct developmental pathways using in vitro and in vivo methods.
Jain, N., Ådén, J., Nagamatsu, K., Evans, M. L., Li, X., McMichael, B. et al. (2017). Inhibition of curli assembly and Escherichia coli biofilm formation by the human systemic amyloid precursor transthyretin. Proceedings of the National Academy of Sciences, 114(46), 201708805.
Smith, D. R., Price, J. E., Burby, P. E., Blanco, L. P., Chamberlain, J., & Chapman, M. R. (2017). The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli. Biomolecules, 7(4).
Friedland, R. P., & Chapman, M. R. (2017). The role of microbial amyloid in neurodegeneration. PLoS Pathog, 13(12), e1006654.
Deshmukh, M., Evans, M. L., & Chapman, M. R. (2018). Amyloid by Design: Intrinsic Regulation of Microbial Amyloid Assembly. J Mol Biol, 430(20), 3631-3641.
Evans, M. L., Gichana, E., Zhou, Y., & Chapman, M. R. (2018). Bacterial Amyloids. Methods Mol Biol, 1779, 267-288.
Price, J. E., & Chapman, M. R. (2018). Phaged and confused by biofilm matrix. Nat Microbiol, 3(1), 2-3.
Klein, R. D., Shu, Q., Cusumano, Z. T., Nagamatsu, K., Gualberto, N. C., Lynch, A. J. L. et al. (2018). Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation. MBio, 9(4).
Hufnagel, D. A., Price, J. E., Stephenson, R. E., Kelley, J., Benoit, M. F., & Chapman, M. R. (2018). Thiol Starvation induces redox-mediated dysregulation of Escherichia coli biofilm components. J Bacteriol, 200(1).