Ph.D. University of Pennsylvania. Bioengineering. 1989.
M.S.E. University of Pennsylvania. Bioengineering. 1985.
B.S.E. Tulane University. Biomedical Engineering. 1983.
Dr. Kohn's research program focuses on two aspects of biomineralization. First, we seek to understand how the organizational hierarchy of mineralized tissues results in functional/mechanical competence (or, conversely, increased susceptibility to fragility). Second, we seek to mimic aspects of Nature's biomineralization strategies as a design basis for developing platforms for regenerative medicine.
Regarding the first aspect of our program, we seek to establish structure-function relations in mineralized tissues and study functional adaptation of these tissues in response to perturbations in the local microenvironment (e.g. alterations in mechanical loading, interactions with biomaterials, disease, aging). There are two guiding principles to our analysis of structure-function relations: it is highly integrated - they develop correlations between mechanical properties, crystal structure/orientation, protein orientation, chemical composition and gene expression; and seek to establish these correlations at different levels of organizational hierarchy - from the whole bone-level down through the tissue and ultrastructural-levels to the protein and gene-levels.
Regarding the second aspect of our program, biomimetic strategies are used to develop systems in which biological output (in-vitro and in-vivo) can be quantitatively related to a well-controlled engineering input. A more long-term, applied and clinically motivated goal is to ultimately utilize this information to develop approaches to replace and/or regenerate tissues. In an effort to create biomaterials that modulate biological response in a controlled manner, we synthesize, characterize and evaluate more biologically based biomaterials. Specifically, this biomimetic approach exploits 3 strategies used by Nature - organic template-mediated self-assembly, targeted adhesion, and cell-cell communication.
Rossello RA, Kohn DH, Gap junction intercellular communication: a review of a potential platform to modulate craniofacial tissue engineering, J Biomed Mater Res, Part B, 88B: 509-518, 2009; PMCID: PMC2744807.
Segvich SJ, Biswas S, Becker U, Kohn DH, Identification of peptides with targeted adhesion to bone-like mineral via phage display and computational modeling, Cells Tissue Organs, 189: 245-251, 2009; PMCID: PMC2739672.
Kohn DH, Sahar ND, Wallace JM, Golcuk K, Morris MD, Exercise alters mineral and matrix composition in the absence of adding new bone, Cells Tissue Organs, 189: 33-37, 2009; PMCID: PMC2824184.
Segvich SJ, Smith HC, Kohn DH, The adsorption of preferential binding peptides to apatite-based materials, Biomaterials, 30: 1287-1298, 2009; PMCID: PMC2744811.
Wallace JM, Golcuk K, Morris MD, Kohn DH, Inbred strain-specific response to biglycan deficiency in the cortical bone of C57BL6/129 and C3H/He mice, J Bone Min Res, 24: 1002-1012, 2009; PMCID: PMC3276349.
Scheller EL, Krebsbach PH, Kohn DH, Tissue engineering: state of the art in oral rehabilitation, J Oral Rehab, 36; 368–389, 2009; PMCID: PMC2744808.
Segvich SJ, Kohn DH, Phage Display as a Strategy for Designing Organic/Inorganic Biomaterials, In: Biological Interactions on Materials Surfaces: Understanding and Controlling Protein, Cell, and Tissue Responses, Ed, R Bizios, DA Puleo, Eds, Springer, New York, pp. 115-132, 2009.
Rossello RA, Wang Z, Kizana E, Krebsbach PH, Kohn DH, Connexin 43 as a signaling platform for increasing the volume and spatial distribution of regenerated tissue, PNAS 106: 13219–13224, 2009; PMCID: PMC2726403.
Luong LN, McFalls KM, Kohn DH, Gene delivery via DNA incorporation within a biomimetic apatite coating, Biomaterials 30: 6996-7004, 2009; PMCID: PMC2783865.
Rossello, RA, Kohn, DH, Cell communication and tissue engineering, Communicative and Integrative Biology, 3: 53-56, 2010; PMCID: PMC2881242.
Wallace JM, Golcuk K, Morris MD, Kohn DH, Inbred strain specific effects of exercise in wild type and biglycan deficient mice, Ann Biomed Engr 38: 1607-1617, 2010; PMCID: PMC2865557.
Addison WN, Miller SJ, Ramaswamy J, Mansouri AM, Kohn DH*, McKee MD*, Phosphorylation of mineral-specific peptide sequences discovered via phage display, Biomaterials 36: 9422-9430, 2010; PMCID: PMC2976791 (* = co-corresponding authors).
Luong LN, Ramaswamy J, Kohn DH, Effects of osteogenic growth factors on bone marrow stromal cell differentiation in a mineral-based delivery system, Biomaterials, 33: 283-294, 2012; PMCID: PMC3208792.
Zhang N, Kohn DH, Using polymeric materials to control stem cell behavior for tissue regeneration, Birth Defects Research Part C: Embryo Today: Reviews, 96: 63-81, 2012; PubMed in process
Ramaraju H, Miller SJ, Kohn DH, Dual-functioning phage derived peptides encourage human bone marrow cell specific attachment to mineralized biomaterials, Connective Tissue Research, 55 Suppl 1: 160-163, 2014; PMCID: PMC4245028.
McNerny EMB, Gong B, Morris MD, Kohn DH, Bone toughness and strength correlate with collagen cross-link maturity in a dose-controlled lathyrism mouse model, J Bone Min Res, 30: 455-464, 2015. PMCID: PMC4333018.
Gardinier JD, Mohamed F, Kohn DH, PTH signaling during exercise contributes to bone adaptation, J Bone Min Res, 30: 1053-1063, 2015. PMC Journal in Progress.
McNerny EM., Gardinier JD, Kohn DH, Exercise increases pyridinoline cross-linking and counters the mechanical effects of concurrent lathyrogenic treatment, Bone, 81: 327-337, 2015. PMC Journal in Process.
Ramaswamy J, Nam HK, Hatch NE, Kohn DH, Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide, Biomaterials, 73: 120-130, 2015; PMCID: PMC4605886.