B.A. University of Minnesota. 1976.
M.D. University of Rochester. 1981.
Fellowship (Infectious Diseases). University of Rochester. 1986.
Postdoctoral Fellowship (Virology). University of North Carolina, Chapel Hill. 1988.
My laboratory has been funded by multiple NIH and foundation grants to study the role of cellular factors in retroviral pathogenesis for more than twenty years. These studies have focused on the interactions between HIV-1, HIV-2, Human Endogenous Retroviruses and other retroviruses with human cells, and therefore the laboratory has also obtained substantial expertise and published multiple papers on aspects of cell biology that emerged from our studies of retroviral pathogenesis. Recently, we obtained a Transformative R01 from the NIH to study endogenous retroviruses, which make up 8% of the human genome. / / My research group has also published extensively on the biochemically distinct DEK oncoprotein, which has been implicated in the pathogenesis of cancer and juvenile arthritis. Our research has recently demonstrated that DEK modulates global heterochromatin integrity and controls the proliferation of melanoma and its response to chemotherapeutic agents. Intriguingly, DEK also has a second life as a secreted, pro-inflammatory protein that is a chemoattractant for neutrophils and lymphocytes, consistent with its apparent role in the pathogenesis of juvenile arthritis. More recently, we have demonstrated that DEK can not only be secreted, but also can be taken up in a bioactive form by adjacent cells. Thus, this paracrine loop might augment the role of DEK in the pathogenesis of cancer and juvenile arthritis, and perhaps influence chromatin biology. / / Another major focus in the laboratory is the development of a banana lectin that inhibits viral replication. We have shown that mutation of a single amino acid in this lectin, termed BanLec, can result in the loss of mitogenicity while retaining antiviral potency. We are now examining the use of modified BanLec as a therapeutic agent against influenza, HIV, and hepatitis C virus. Thus, our basic science studies of viruses and the cellular proteins that interact with them have led us into extremely interesting mechanistic studies of viral and cell biology, as well as pointing the way towards the development of new therapeutic agents.
Mor-Vaknin, N., Kappes, F., Dick, A.E., Legendre, M., Damoc, C., Teitz-Tennenbaum, S., Kwok, R., Ferrando-May, E., Adams, B.S., and Markovitz, D.M. DEK in the synovium of JIA patients: characterization of DEK antibodies and posttranslational modification of the DEK autoantigen. Arthritis & Rheumatism 63(2):556-567, 2011. PMC3117121
Kappes, F., Waldmann, T., Mathew, V., Yu, J., Zhang, L., Khodadoust, M.S., Chinnaiyan, A.M., Luger, K., Erhardt, S., Schneider, R., and Markovitz, D.M. The DEK oncoprotein is a su(var) that is essential to heterochromatin integrity. Genes & Development 25:673-678, 2011. PMC3070930
Stelzer, A.C., Frank, A. T., Kratz, J.D., Swanson, M., Gonzalez-Hernandez, M.J., Lee, J., Andricioaei, I., Markovitz, D.M., and Al-Hashimi, H.M. Discovery of selective bioactive small molecules by targeting an RNA dynamic ensemble. Nature Chemical Biology 7(8):553-9, 2011. PMC3319144
Gonzalez-Hernandez, M. J., Swanson, M. D., Contreras-Galindo, R., Cookinham, S., King, S. R., Noel, R. J. Jr., Kaplan, M. H., and Markovitz, D. M. Expression of Human Endogenous Retrovirus Type-K (HML-2) is activated by the Tat protein of HIV-1. J. Virol. (epub ahead of print May 16, 2012).
Contreras-Galindo, R.A., Kaplan, M.H., Contreras-Galindo, A.C., Gonzalez-Hernandez, M., Ferlengui, I., Giusti, F., Lorenzo, E., Gitlin, S.D., Dosik, M.H., Yamamura, Y., and Markovitz, D.M. Characterization of human endogenous retroviral elements in the blood of HIV-1-infected individuals. J. Virol. 86(1):262-76, 2012. PMC3255917