Julie Suzanne Biteen is currently an Assistant Professor of Chemistry at the University of Michigan. She is the recipient of a National Science Foundation CAREER Award and a Burroughs Wellcome Fund Career Award at the Scientific Interface. She obtained her Ph.D. from California Institute of Technology in 2006 with Professors Harry Atwater and Nathan Lewis and was a postdoctoral scholar at Stanford University with Professor W.E. Moerner. Her research program applies super-resolution microscopy and plasmon-enhanced fluorescence to outstanding questions in microbiology and nanotechnology.
By beating the diffraction limit that restricts traditional light microscopy, single-molecule fluorescence imaging is a flexible, noninvasive way to sensitively probe position and dynamics.
Our lab has pioneered super-resolution imaging methods for applications to central processes that remain contested by microbiologists. Based on the methods that we have developed forsingle-molecule imaging in live bacteria cells, we have uncovered the organization of structural proteins, we have revealed the mechanism of membrane-bound transcription regulation in the pathogenic Vibrio cholerae, we have demonstrated the dynamic interactions involved in starch degradation in the human gut symbiont Bacteroides thetaiotaomicron, and we have uncovered an intimate and dynamic coupling between DNA mismatch recognition and DNA replication in a highly conserved repair pathway.
Moreover, we have examined the fundamentals of plasmon-enhanced fluorescence with a combination of single-molecule imaging experiments and electromagnetic simulations, and we are now applying this understanding to biocompatible enhancement of fluorescent protein emission, extending the implications of metal-enhanced fluorescence to increased signal and resolution in live-cell bio-imaging, and creating a flexible technology for high-resolution, real-time imaging.
B.L. Haas, J.S. Mason, V.J. DiRita and JSB, “Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter,” Molecular Microbiology, in press (2014). http://dx.doi.org/10.1111/mmi.12834
K.S. Karunatilaka, E.A. Cameron, E.C. Martens, N.M. Koropatkin and JSB, “Super-Resolution Imaging Captures Carbohydrate Utilization Dynamics in Human Gut Symbionts,” mBio, 5 e02172-14 (2014). http://dx.doi.org/10.1128/mBio.02172-14
J.E. Donehue, E. Wertz, C.N. Talicska and JSB, “Plasmon-Enhanced Brightness and Photostability from Single Fluorescent Proteins Coupled to Gold Nanorods,” Journal of Physical Chemistry C, 118 15027-15035 (2014).
M.-C. Cheng, A. T. Leske, T. Matsuoka, B. C. Kim, J.-S. Lee, M. A. Burns, S. Takayama and JSB, “Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy,” Journal of Physical Chemistry B, 117 4406-4411 (2013).
Y. Liao, S.K. Yang, K. Koh, A.J. Matzger and JSB, “Heterogeneous Single-Molecule Diffusion in One- Two- and Three-Dimensional Microporous Coordination Polymers: Directional, Trapped and Immobile Guests,” Nano Letters, 12 3080 (2012).