Ph.D. Stanford University. Materials Science and Engineering. 1998.
M.S. Stanford University. Materials Science and Engineering. 1994.
B.S. University of Tokyo. Precision Engineering. 1992.
The primary objective of my project is to develop a revolutionary, easy-to-implement, integrated microfluidic platform for multi-analyte functional immunophenotyping assay for leukocytes isolated from whole blood. The proposed technique is to overcome major drawbacks of currently available methods for immunoparalysis detection of pediatric patients. This research project is to be highly interdisciplinary and carried out in collaboration with PIs at CoE and Med. School. My research team plans to lead the effort to design a microfluidic immunophenotyping assay (MIPA) device and to develop an optical setup that enables on-chip multi-parametric detection of cell-secreted cytokines in the MIPA platform. My additional role is to calibrate, analyze, and validate the immunoassay signal data through their comparison with results of the ELISA-based standard immunoassay. I have a strong research track record in developing new optical microsystems technology for microfluidic and lab-on-a-chip applications using microelectromechanical systems (MEMS) technology. As PI on several NSF-, DoD-, and NIH-funded grants, I have led research efforts for a nearly decade, leading to the development of optical systems that provide an experimental platform for microfluidic protein binding assays, multispectral detection of color-coded bioparticles, and in-vivo optical molecular imaging for colon cancer detection. I have successfully published journal papers that provide significant insights into future miniaturization of the optical reading system for biophotonic applications.
Y.-C. Tung, N.-T. Huang, B.-R. Oh, B. Patra, C.-C. Pan, T. Qiu, P.K. Chu, W. Zhang, and K. Kurabayashi, “Optofluidic Detection for Cellular Phenotyping,” Lab Chip, 12, 3552-3565 (2012).
N.-T. Huang, W. Chen, B.-R. Oh, T.T. Cornell, T.P. Shanley, J. Fu, and K. Kurabayashi, “An Integrated Microfluidic Platform for In-situ Cellular Cytokine Secretion,” Lab Chip, 12, 4093-4101 (2012).
N.-T. Huang, S.C. Truxal, Y.-C. Tung, A.Y. Hsiao, G.D. Luker, S. Takayama, and K. Kurabayashi, “Multiplexed Spectral Signature Detection for Microfluidic Color-Coded Bioparticle Flow,” Anal. Chem., 82, 9506-9512 (2010).
C.T Lin, E. Meyhofer, and K. Kurabayashi, “Predicting the stochastic guiding of kinesin-driven microtubules in microfabricated tracks: A statistical-mechanics-based modeling approach,” Phys. Rev. E., 81, 011919 (2010).
N.-T. Huang, S.C. Truxal, Y.-C. Tung, A. Hsiao, S. Takayama, and K. Kurabayashi, “High-speed tuning of visible laser wavelength using a nanoimprinted grating optical tunable filter,” Appl. Phys. Lett., 95, 211106 (2009).
C.T. Lin, M.T. Kao, E. Meyhofer, and K. Kurabayashi, “Surface Landing of Microtubule Nanotracks Influenced by Lithographically Patterned Channels,” Appl. Phys. Lett., 95, 103701 (2009).
S.J. Kim, F. Wang, M.A. Burns, and K. Kurabayashi, “Temperature-Programmed Natural Convection for Micromixing and Biochemical Reaction in a Single Microfluidic Chamber,”Anal. Chem., 81, 4510-4516 (2009).
C.-T. Lin, M.-T-. Kao, K. Kurabayashi, and E. Meyhofer, “Self-contained biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip,” Nano Lett. 8, 1041-1046 (2008).
C.Y. Fan, Y.-C. Tung, S. Takayama, E. Meyhofer, and K. Kurabayashi, “'Electrically Programmable Surfaces for Configurable Patterning of Cells," Adv. Mater., 20, 1418-1423 (2008).
C.Y. Fan, K. Kurabayashi, and E. Meyhofer, “Protein Pattern Assembly by Actively Controlling a Triblock Copolymer Monolayer,” Nano Lett., 6, 2763-2767 (2006).