Professor Takayama's research group focuses on constructing microscale models of the body. Much of our body is comprised of small vessels, tubes, ducts and channels. In other words, our body is a sort of microfluidic circuit. The Takayama lab borrows tools from the microelectronics industry to create man-made microfluidic circuitry with living cells inside of them. The lab also utilizes aqueous two phase systems and nanochannels created by fracture fabrication to analyze proteins, disease biomarkers, DNA, and chromatin. Specific applications include construction of artificial blood vessels to study breast cancer metastasis, generation of engineered small airways to study lung disease, and enhancing in vitro fertilization treatments by making artificial oviducts to manipulate sperm, eggs, and embryos. The lab also develop tools for high throughput drug testing and multiplexed disease biomarker assays. More fundamental research such as dissecting cell signaling networks, analyzing cell chemotaxis, and manipulating bacterial communities is also performed utilizing microfluidic technologies.
Mehta, G.; Hsiao, A.Y.; Ingram, M.; Luker, G.D.; Takayama, S. "Opportunities and challenges for use of tumor spheroids as models to test drug delivery efficacy." J. Controlled Release. 2012 DOI: 10.1016/j.jconrel.2012.04.045.
Wang, J.D.; Douville, N.J.; Takayama, S.; El-Sayed, M. "Quantitative Analysis of Molecular Absorption into PDMS Microfluidic Channels." Annals of Biomedical Engineering. 2012 DOI: 10.1007/s10439-012-0562-z.
Heo, Y.S.; Cabrera, L.M.; Bormann, C.L.; Smith, G.D.; Takayama, S. "Real time culture and analysis of embryo metabolism using a microfluidic device with deformation based actuation." Lab Chip. 2012 DOI: 10.1039/C2LC21050A.
Fang, Y.; Frampton, J.P.; Raghavan, S.; Savahi-Kaviani, R.; Luker, G.; Deng, C.X.; Takayama, S. "Rapid Generation fo Multiplexed Cell Cocultures Using Acousitc Droplet Ejection Followed by Aqueous Two-Phase Exlcusion Patterning." Tiss. Eng. C. 2012. DOI: 10.1089/ten.tec.2011.0709.
Kim,S.-J.; Yokokawa, R.; Lesher-Perez, S.C.; Takayama, S. "Constant flow-driven microfluidic oscillator for different duty cycles." Anal Chem 2011. DOI: 10.1021/ac202866b.
Zamankhan, P.; Helenbrook, B.T.; Takayama, S.; Grotberg, J.B. "Steady motion of Bingham liquid plugs in two-dimensional channels." J Fluid Mech. 2011. DOI: 10.1017/jfm.2011.43.