Dr. Dou's broad objectives are to study the chromatin modifying enzymes that catalyze histone lysine methylation, acetylation and ubiquitylation and develop inhibitory molecular probes that allow for functional studies. We are currently studying the regulation of the MLL/SET1 family histone H3 lysine 4 methyltransferases and MYST family histone acetyltransferase MOF, two enzymes that function coordinately in transcription activation. We are also characterizing the molecular mechanisms of how these enzymes contribute to malignant processes in vivo.
Wu L, Lee SY, Zhou B, Nguyen UT, Muir TW, Tan S, Dou Y: ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes. Molecular Cell, 49 (6): 1108-1120, 2013.
Cao F, Townsend EC, Karatas H, Xu J, Li L, Lee S, Liu L, Chen Y, Ouillette P, Zhu J, Hess JL, Atadja P, Lei M, Qin ZS, Malek S, Wang S, Dou Y: Targeting MLL1 H3K4 methyltransferase activity in mixed-lineage leukemia. Molecular cell, 53 (2): 247-61, 2014.
Wu L, Li L, Qin Z and Dou Y: MSL2 mediated H2B K34 ubiquitylation promotes RNA Pol II processivity through regulating PAF1 and pTEFb pathways. Molecular Cell, 54 (6): 920-931, 2014.